The Cell Total/Animal Total RNA Isolation Kit is a product developed by Foregene, a company that specializes in life science research products. The kit employs centrifugal columns and formulations that simplify the RNA isolation process while ensuring high yields and purity. Additionally, this kit comes with a DNA-Cleaning column that separates the supernatant from lytic cell content and removes genomic DNA.
The RNA isolation procedure using the Cell Total/Animal Total RNA Isolation Kit involves four main steps. First, cells must be homogenized in an appropriate buffer to disrupt the cell membrane and release the RNA. Second, the lysate is treated with a DNAse to remove any genomic DNA. Third, the RNA is bound to the RNA-binding column with the aid of a specially-formulated buffer. Finally, the RNA is eluted from the column with a low ionic strength elution buffer.
One of the advantages of the Cell Total/Animal Total RNA Isolation Kit is its simplicity. The protocol is straightforward and can be completed in less than an hour, depending on the sample size. The DNA-Cleaning column effectively removes genomic DNA, eliminating the need for additional treatments and saving time. The kit is also compatible with various samples from different sources, including animal tissues and cultured cells.
Another significant advantage of the Cell Total/Animal Total RNA Isolation Kit is the quality of RNA produced. The RNA-only column efficiently binds to RNA molecules, minimizing the loss of RNA during the isolation process. The RNA obtained is pure and free from impurities that could interfere with downstream analyses. This high-quality RNA is suitable for various applications requiring intact RNA, such as microarray hybridizations, gene expression profiling, qPCR, and sequencing.
The use of RNA isolation kits, such as the Cell Total/Animal Total RNA Isolation Kit, has become increasingly popular over the years. These kits offer a convenient and efficient way to extract high-quality RNA, saving time and resources. Furthermore, the use of kits eliminates the need for specialized training and equipment, making RNA isolation more accessible to researchers without extensive molecular biology expertise.
In conclusion, the Cell Total/Animal Total RNA Isolation Kit is an excellent option for researchers looking for a simple and efficient way to isolate high-quality RNA. This kit offers several benefits, including a quick and straightforward protocol, effective removal of genomic DNA, and efficient RNA binding. Moreover, the kit produces RNA that is suitable for various applications, making it a versatile tool for researchers. For optimal results, researchers can combine the Cell Total/Animal Total RNA Isolation Kit with other tools, such as the Mirvana RNA Isolation Kit, for better yields and purity.