RNA isolation is a critical step in many molecular biology experiments that require high-quality RNA samples, such as gene expression analysis, transcriptome sequencing, and RNA interference (RNAi) studies. Total RNA isolation kits are designed to extract RNA from a wide range of biological samples, including cells, tissues, and bodily fluids. In this blog post, we'll discuss the Cell Total/Animal Total RNA Isolation Kit, a high-performance RNA isolation kit that utilizes centrifugal columns and advanced formulas developed by Foregene.
What is RNA Isolation?
RNA isolation is the process of extracting total RNA from biological samples for downstream applications. Total RNA is a mixture of mRNA, tRNA, rRNA, and other small RNA molecules, and its isolation requires careful handling to avoid RNA degradation and contamination. RNA isolation methods may vary depending on the sample type, the application, and the desired RNA quality and quantity.
The Cell Total/Animal Total RNA Isolation Kit
The Cell Total/Animal Total RNA Isolation Kit is a reliable and efficient RNA isolation kit designed to extract high-quality RNA from various animal cells and tissues. This kit is based on silica-based centrifugal columns that allow for efficient binding and elution of RNA molecules. The kit includes a DNA-Cleaning column that removes genomic DNA from RNA samples, ensuring pure RNA samples for downstream applications.
The key features of the Cell Total/Animal Total RNA Isolation Kit include:
- Efficient RNA binding and elution: The kit utilizes centrifugal columns that allow for efficient RNA binding and elution, ensuring high RNA yield and quality.
- DNA removal: The DNA-Cleaning column enables efficient removal of genomic DNA from RNA samples, minimizing contamination and ensuring pure RNA samples.
- Unique buffer: The kit includes a uniquely formulated buffer that facilitates efficient RNA binding to the column, further increasing RNA yield and quality.
- Compatibility: The kit is compatible with a wide range of animal cells and tissues, including cultured cells, fresh or frozen tissues, blood, and plasma.
The RNA isolation procedure using the Cell Total/Animal Total RNA Isolation Kit is simple and time-efficient, requiring only a few steps to get high-quality RNA samples. Here's a general overview of the RNA isolation protocol using this kit:
1. Collect the animal cells or tissues and homogenize them in the lysis buffer provided in the kit. The homogenization can be performed mechanically or manually, depending on the sample type.
2. Transfer the lysate to the DNA-Cleaning column and spin it down to remove genomic DNA.
3. Transfer the flow-through to the RNA binding column and centrifuge it to bind the RNA to the column.
4. Wash the RNA-bound column with the washing buffer to remove any residual contaminants.
5. Elute the RNA from the column using RNase-free water or elution buffer provided in the kit.
The resulting RNA sample can be used for downstream applications, such as RT-PCR, microarray analysis, RNA sequencing, and more.
Conclusion
In this blog post, we discussed the Cell Total/Animal Total RNA Isolation Kit, a reliable and efficient RNA isolation kit that utilizes centrifugal columns and advanced formulas for high-quality extraction of RNA from animal cells and tissues. RNA isolation is a crucial step in many molecular biology experiments, and choosing the right RNA isolation kit is essential for obtaining high-quality RNA samples for downstream applications. The Cell Total/Animal Total RNA Isolation Kit offers a simple, time-efficient, and reliable RNA isolation method, making it a popular choice for researchers in the field.