Total RNA is a type of RNA that is extracted from cells or tissues that contain all the RNA species present in the sample. It includes messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA). Total RNA is an essential starting material for many molecular biology techniques, including qPCR.
References and Controls are included in qPCR experiments to ensure that the assay is reliable and consistent. Reference RNAs are used as a standard for the assay, and help to control for variation in RNA isolation and cDNA synthesis. Controls are used to monitor the efficiency of the PCR reaction and to detect potential contamination.
Primers are short sequences of DNA that are complementary to the target sequence of the RNA molecule of interest, and serve as a starting point for the PCR reaction. Reverse primers are used to generate cDNA from the RNA template, while forward primers are used in the PCR reaction to amplify the cDNA. Therefore, primers are essential components of qPCR assays.
In qPCR experiments, a Universal Reverse Primer is a useful tool to have, as it can be used in conjunction with multiple forward primers to amplify different RNA transcripts. This simplifies the experimental design and reduces the cost of primer synthesis.
Passive reference dyes are also used in qPCR assays, and are included in the reaction mix to monitor the reaction efficiency. One such dye is Rox, which is a passive reference dye that does not interact with the PCR reaction, but emits fluorescence at a different wavelength from the fluorescent reporter dye used to monitor the amplification.
The Alien Reference RNA QRT-PCR Detection Kit is a novel qPCR kit that is designed to detect RNA from a wide range of species, including human, rat, and mouse. It is a highly sensitive and specific assay, and is ideal for researchers who work with RNA from multiple species.
MVP Total mouse and rat RNA is another useful reference RNA, as it is a mixture of RNA from multiple tissues in both mice and rats. It is a convenient starting material for qPCR experiments, as it contains a variety of RNA species that are representative of the entire organism.
In conclusion, Total RNA, References & Primers are essential components of the qPCR assay, and are important for ensuring accurate and reliable results. The Universal Mouse Total RNA is an important keyword to remember when searching for these products, as it is a common starting material for many qPCR experiments. By using high-quality references and controls, researchers can ensure that their qPCR experiments are consistent and produce reliable data.