Quantitative polymerase chain reaction (qPCR) has become one of the most widely used methods for gene expression analysis as it provides accurate quantification of RNA targets. In qPCR, the real-time quantification is done by measuring the fluorescence produced during the amplification of the specific target DNA sequence. To achieve high-quality results, it is essential to select an appropriate qPCR reagent that can detect and amplify even low-abundance RNA targets.
There are a variety of qPCR reagents available on the market, but not all of them offer the same level of performance. One of the best options for reliable and ultrasensitive qPCR is the (remove brand name) Brilliant II SYBR Master Mix.
This next-generation qPCR master mix reagent is specifically designed for reliable quantification across a wide range of targets and templates. The Brilliant II SYBR Master Mix can detect low-copy number targets with exceptional sensitivity, providing reliable quantification across a broad dynamic range that can span up to nine orders of magnitude.
One of the standout features of the Brilliant II SYBR Master Mix is its high level of specificity. This qPCR reagent utilizes a unique synthetic DNA-binding fluorescent dye with enhanced specificity for double-stranded DNA. This dye interacts solely with double-stranded DNA and generates a robust and reproducible fluorescent signal that is proportional to the amount of amplified target DNA, leading to highly reliable and accurate results.
Another advantage of the Brilliant II SYBR Master Mix is its flexibility in working with a variety of sample sources, including cDNA, genomic DNA, and plasmid DNA. The reagent is compatible with most qPCR instruments and performs consistently and reproducibly across different qPCR platforms.
In addition, the Brilliant II SYBR Master Mix also offers a number of other benefits that make it an excellent choice for performers of SYBR Green qPCR assays. The reagent contains a highly rigid and ultra-pure buffer formulation, along with an innovative hot start Taq DNA polymerase, making it possible to perform robust amplification of even challenging templates. The hot start mechanism helps to minimize background amplification by preventing the polymerase from initiating primer extension at low temperatures.
Overall, when using the (remove brand name) Brilliant II SYBR Master Mix, researchers can achieve highly sensitive and specific qPCR results with reliable quantification and low background levels. This makes it an essential reagent for gene expression analysis, diagnostics, and research.
In conclusion, if you are looking for a qPCR reagent that can provide highly sensitive and reliable quantification across a broad dynamic range of RNA targets, the Brilliant II SYBR Master Mix is an excellent choice. Its robust performance, flexibility, and innovative hot start mechanism make it an essential tool for gene expression analysis, diagnostics, and research. Always remember that choosing the right qPCR reagent is the first and most important step towards achieving accurate and reliable qPCR results.