Products Details
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns■ No time-consuming and expensive DNA purification ■ The sample demand is small, as little as 5mg animal tissue can be tested. ■ No special treatment such as grinding and crushing is required, and the operation is simple. ■ Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.
Application: a variety of animal tissues. DNA released from sample lysis: only used as a PCR template. The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc. | Part I | Buffer AL |
| Foregene Protease |
| 6× DNA Loading Buffer |
| Part II | 2× PCR EasyTM Mix |
Part I of this kit should be stored at 2-8℃. The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage. Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months. Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time. Part II of this kit should be stored at -20℃ . Reagent 2×PCR EasyTM Mix can be stored for 12 months at -20℃ ; if it is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days). This kit uses a unique lysis buffer system to quickly release genomic DNA from animal tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing. The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction. 2× PCR EasyTM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer. D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.
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