50 Preps, 200 Preps This kit uses the spin column and formula developed by our company, which can extract high-purity and high-quality total RNA from various animal tissues with high efficiency.It provides an efficient DNA-Cleaning Column, which can easily separate and adsorb genomic DNA from the supernatant and tissue lysate, simple and time-saving; RNA-only Column can efficiently bind RNA and can be processed simultaneously with a unique formula Lots of samples. The whole system is RNase-Free, so that the extracted RNA is not degraded; Buffer RW1, Buffer RW2 buffer washing system, so that the obtained RNA is free of protein, DNA, ion, and organic compound pollution.

Animal Total RNA Isolation Kit
Kit components	RE-03011	RE-03014
	50 T	200 T
Buffer RL1*	25ml	100ml
Buffer RL2	15ml	60ml
Buffer RW1*	25ml	100ml
Buffer RW2	24ml	96ml
RNase-Free ddH2O	10ml	40ml
RNA-only Column	50	200
DNA-Cleaning Column	50	200
Instruction Manual	1 piece	1 piece

 

Format	Spin column	Purification component	Foregene column, reagent
Flux	1-24 samples	Time per prep	~30 min (24 samples)
Centrifuge	Desk centrifuge	Pyrolysis separation	Centrifugal separation
Sample	Animal tissue; cell	Samples amount	Tissue:10-20 mg; Cell:(1-5)×106
Elution volume	50-200 μL	Maximum loading volume	850 μL

 ■ No need to worry about RNA degradation; the whole system is RNase-Free ■ Effectively remove DNA-using DNA-Cleaning Column ■ Remove DNA without adding DNase ■ Simple-all operations are completed at room temperature ■ Fast -operation can be completed in 30 minutes ■ Safe-no organic reagent required ■ High purity -OD260/280≈1.8-2.1It is suitable for the extraction and purification of total RNA from a variety of fresh or frozen animal tissues or cultured cells.■ Downstream applications: first-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, etc. ■ Samples: animal tissues, cultured cells ■ Dosage: Tissues 10-20mg, Cells(2-5)×10⁶ ■ Maximum DNA binding capacity of purification column: 80 μg ■ Elution volume: 50-200 μlAnimal Total RNA Isolation Kit treated 20mg Fresh mouse samples, take 5% purified total RNA 1% agar Glycogel electrophoresis 1: Spleen 2: Kidney 3: Liver 4: HeartThe kit can be stored for 24 months at room temperature (15–25 ℃) or 2–8 ℃ for longer time. Buffer RL1 can be stored at 4 ℃ for 1 month after adding β- mercaptoethanol (optional).1. IF:18.808: Zheng, Q., Qin, F., Luo, R., et al. mRNA-Loaded Lipid-Like Nanoparticles for Liver Base Editing Via the Optimization of Central Composite Design. Adv. Funct. Mater. 2021, 31, 2011068.doi:10.1002/adfm.202011068. 2.IF:18.187:He X, Hong W, Yang J, et al. Spontaneous apoptosis of cells in therapeutic stem cell preparation exert immunomodulatory effects through release of phosphatidylserine. Signal Transduct Target Ther. 2021 Jul 14;6(1):270. doi: 10.1038/s41392-021-00688-z. 3.IF:17.97:Dai Z, Liu H, Liao J, et al. N7-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression. Mol Cell. 2021 Jul 29:S1097-2765(21)00555-4. doi: 10.1016/j.molcel.2021.07.003. 4.IF:9.225:Cao X, Shu Y, Chen Y, et al. Mettl14-Mediated m6A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis. Cell Mol Gastroenterol Hepatol. 2021;12(2):633-651. doi: 10.1016/j.jcmgh.2021.04.001.   RNA isoaltion kits for other sample sources are available: Cell, plant, viral, blood, etc.