Factory Direct: Get Accurate QPCR Results With Cell Direct RT Kits

Cell Direct RT qPCR Kit---Taqman Direct Cell Lysis Cell Ready One-step qRT-PCR Kits Probe

Cat.No.DRT-01021/01022 For cell direct RT-qPCR using ≤ 1000,000 cells, and performing RT qPCR directly from cells without prior RNA purification. Cells are lysed directly to release RNA for RT-qPCR; the high tolerance system makes it unnecessary to purify RNA and directly use cell lysates as RNA templates for RT reactions. Fast and convenient; high sensitivity, strong specificity, and good stability. ◮Simple and effective: with Cell Direct RT technology, RNA samples can be obtained in just 7 minutes. ◮The sample demand is small, as low as 10 cells can be tested. ◮High throughput: it can quickly detect RNA in cells cultured in 384, 96, 24, 12, 6-well plates. ◮DNA Eraser can quickly remove released genomes, greatly reduce the impact on subsequent experimental results. ◮Optimized RT and qPCR system makes the two-step RT-PCR reverse transcription more efficient and PCR more specific, and more resistant to RT-qPCR reaction inhibitors.

■ Simple and effective : with Cell Direct RT technology, RNA samples can be obtained in just 7 minutes. ■ The sample demand is small, as low as 10 cells can be tested. ■ High throughput: it can quickly detect RNA in cells cultured in 384, 96, 24, 12, 6-well plates. ■ DNA Eraser can quickly remove released genomes, greatly reduce the impact on subsequent experimental results. ■ Optimized RT and qPCR system makes the two-step RT-PCR reverse transcription more efficient and PCR more specific, and more resistant to RT-qPCR reaction inhibitors.Scope of application: cultured cells. - RNA released by sample lysis: only applicable to the RT-qPCR template of this kit. - The kit can be used for the following purposes: gene expression analysis, verification of siRNA-mediated gene silencing effect, drug screening, etc.This product uses a unique lysis buffer system to quickly release RNA from cultured cell samples for RT-qPCR reactions, eliminating the time-consuming and laborious RNA purification process, and only 7 minutes to obtain the required RNA template, with the 5× Direct RT Mix, 2× Direct qPCR Mix-Taqman provided by the kit can quickly and efficiently obtain real-time quantitative PCR results. 5× Direct RT Mix and 2× Direct qPCR Mix-Taqman have strong inhibitor tolerance and can perform efficient reversal and specific amplification using the lysate of the sample to be measured as a template. The reagent contains Foregene Reverse Transcriptase, Hot D-Taq DNA Polymerase, dNTPs, MgCl₂, Reaction Buffer, PCR Optimizer and Stabilizer, which can be used with lysis buffer to quickly and easily detect samples, and has the characteristics of high sensitivity, specificity and stability.Part I of this kit should be stored at 4℃; Part II should be stored at -20℃. Foregene Protease Plus II should be stored at 4℃,do not freeze at -20℃. Reagent 2×Direct qPCR Mix-Taqman should be stored at -20℃ in the dark; if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).200×20μl Rxns, 1000×20μl Rxns

QuickEasy^TM Cell Direct RT-qPCR Kit-Taqman
Kit components 20μl qPCR Reaction System		DRT-01021	DRT-01022	Note
Kit components 20μl qPCR Reaction System		200 T	1000 T	Note
Part I	Buffer CL	4 ml	20 ml	Cell Lysis
	Foregene Protease Plus II	80 μl	400 μl
	Buffer ST	400 μl	1 ml × 2
Part II	DNA Eraser	80 μl	400 μl
	5×Direct RT Mix *	160 μl	800 μl	RT
	2× Direct qPCR Mix-Taqman *	1 ml × 2	1.7 ml × 6	qPCR
	20×ROX Reference Dye	40 μl	200 μl	qPCR
	RNase-Free ddH₂O	1.7 ml	10 ml
Instruction Manual		1 piece	1 piece

*:Cell Lysis, 5×Direct RT Mix, 2× Direct qPCR Mix-Taqman can be purchased separately

Cell Direct RT qPCR Kit---Taqman Direct Cell Lysis Cell Ready One-step qRT-PCR Kits Probe

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