Get Accurate Results With ForeDirect RT-qPCR Kit

ForeDirect RT-qPCR Kit

◮ For RNA direct real-time amplification from swab collections without RNA purification processes. This kit completes the qRT-PCR cycles within one hour. The enzyme mix is an optimized blend of Reverse Transcriptase, Hot-Start Taq DNA polymerase, RNase inhibitor. The Reaction Buffer contains all of the required components, including optimized buffer components, Mg²⁺, dUTP, and dNTPs.

100T1. Once the PCR reaction tubes have been briefly centrifuged, place them in the sample tank of amplification instrument. 2. Thermal protocol

Step		Temperature	Time	Cycles
1	Reverse transcription	50℃	15 mins	1
2	Pre-denaturation	95℃	1 min	1
3	Denaturation	95℃	10 seconds	42
4	Anneal/Extension	60℃	30 seconds	42

Note: Fluorescent signal was detected immediately after the extension step of each cycle. 3. After setting, save the file and run the reaction program.

Nucleic acid release agent

RNA protectant

2× RT-qPCR Buffer

Enzyme Mixture(Taq&M-MLV)

Instructions

-The kit should be stored at -20 ± 5℃. The validity period is 12 months. -Avoid repeated freeze-thaw cycles (<5 cycles).Before reagent preparation, all the reagents in the kit should be thawed at room temperature and mixed gently. Reagent preparation 1. Prepare the Nucleic Acid Release Mix

Component	Volume per Sample or Control
Nucleic acid release agent	5μL
RNA protectant	0.5 μL

2. Prepare the Reaction Mix

Component	Volume per Sample or Control
2× RT-qPCR Buffer	15 μL
Enzyme Mixture(Taq&M-MLV)	1.5 μL
Primers and Probes	1μL

ForeDirect RT-qPCR Kit is designed for RNA direct real-time amplification from swab collections without RNA purification processes. This kit completes the qRT-PCR cycles within one hour. The enzyme mix is an optimized blend of Reverse Transcriptase, Hot-Start Taq DNA polymerase, RNase inhibitor. The Reaction Buffer contains all of the required components, including optimized buffer components, Mg²⁺, dUTP, and dNTPs.

ForeDirect RT-qPCR Kit

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