Mouse Tail Direct PCR Kit Direct PCR Lysis Reagent(Mouse Tail)(for Genotyping)

Directly use mouse tail lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity.

◮No time-consuming and expensive DNA purification is required.

◮The sample demand is small, just take a single seed.

◮No special treatments such as grinding and crushing are required, and the operation is simple.

◮The optimized PCR system enables PCR to have higher specificity and stronger tolerance to PCR reaction inhibitors.

foregene strength

send email to us

Products Details

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns■ No time-consuming and expensive DNA purification is required. ■ The sample demand is small, as little as 5mg mouse tissue or 2-5mm mouse tail can be tested. ■No special treatments such as grinding and crushing are required, and the operation is simple. ■ The optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.Application: Mouse tail, mouse ears, muscle and other tissues. DNA released from sample lysis: only used as a PCR template. The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.

Part I

Buffer MP

Foregene Protease Plus

6× DNA Loading Buffer

Part II

2× M1 PCR EasyTM Mix

Instructions
Part I of this kit should be stored at 2-8℃. The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃  for longer storage.  Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.  Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time. Part II of this kit should be stored at -20℃.  Reagent 2×M1 PCR EasyTM Mix, if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).This kit uses a unique lysis buffer system to quickly release genomic DNA from mouse tails, ears, muscles and other tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing. The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction. 2× M1-PCR EasyTM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer. D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.  

Previous:MALT1 Dual Color Break Apart probe

Next:TP53/CSP17 Dual Color Probe

Multiplex Kit, Pcr Machine, Saliva Test Kit For Blood Type, Rna Rn112, China PCR Single Tube, Nucleic Acid Kit, Real Time Pcr Advantages, Racked Pipette Tip, Rtpcr Probe, cfDNA isolation kits,

Featured Products

Contact us

Please feel free to give your inquiry in the form below We will reply you in 24 hours