Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Mouse Tail)(for Genotyping)

Directly use mouse tail lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity.

No time-consuming and expensive DNA purification is required.

The sample demand is small, just take a single seed.

No special treatments such as grinding and crushing are required, and the operation is simple.

The optimized PCR system enables PCR to have higher specificity and stronger tolerance to PCR reaction inhibitors.

foregene strength

Products Details

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns■ No time-consuming and expensive DNA purification is required. ■ The sample demand is small, just take mouse tails (or mouse ears with a diameter of 2-5mm) above 2mm. ■ Easy to operate, it can be lysed without heating to inactivate the protease, and the lysed product can be directly subjected to PCR reaction. ■ Foregene Mouse Tail Genomic DNA Extraction Kit (DE-05211) can be used to extract genomic DNA from the processed rat tail. ■ Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.Application: Mouse tail, mouse ears, muscle and other tissues. DNA released from sample lysis: only used as a PCR template. The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.

Part I

Buffer NP

Foregene Protease Plus I

6× DNA Loading Buffer

Part II

2× M PCR EasyTM Mix

Instructions

Part I of this kit should be stored at 2-8℃. The reagent Buffer FP can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage. Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months. Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time. Part II of this kit should be stored at -20℃. Reagent 2×PCR EasyTM Mix, if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).This kit uses a unique lysis buffer system to quickly release genomic DNA from mouse tail and mouse ear samples for PCR reaction, so it is particularly suitable for large-scale genetic testing. The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at room temperature (20-25°C). No heating is required, and other processes such as protein and RNA removal are not required. The released trace amounts of DNA can be used as Template for PCR reaction. 2×M-PCR EasyTM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer. D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

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