Plant leaf Direct PCR plus kit-UNG (without Sampling Tools) Protocol Direct PCR from Plant Material

Directly use polysaccharide and polyphenol leaf lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity. The UNG anti-pollution system has been added to prevent false positives. No time-consuming and expensive DNA purification(Time, labor and cost saved!) Less material Simple—the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding 2 X Mix—reducing sample loading errors and reaction system preparation time Fast—template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes High throughput—lysis reaction can be completed in 96-well PCR plate foregene strength

Products Details

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Part I

Buffer P1

Buffer P2

6×DNA Loading Buffer

Part II

2× Leaf PCR EasyTM Mix(UNG)

■ No time-consuming and expensive DNA purification ■ Less material ■ Simple-the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding ■ 2 × Mix, reducing sample loading errors and reaction system preparation time ■ Fast-template preparation can be completed in 10 minutes, PCR reaction can be completed in at least 50 minutes ■ High throughput-lysis reaction can be completed in 96-well PCR plateApplication: genetically modified identification, genotyping, etc. Sample: plant leaf Dosage: Diameter 2-3mm (direct method), diameter 5-7mm (lysis method) Detection range: target fragment ≤1kb50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Part I

Buffer P1

Buffer P2

6×DNA Loading Buffer

Part II

2× Leaf PCR EasyTM Mix(UNG)

■ No time-consuming and expensive DNA purification ■ Less material ■ Simple-the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding ■ 2 × Mix, reducing sample loading errors and reaction system preparation time ■ Fast-template preparation can be completed in 10 minutes, PCR reaction can be completed in at least 50 minutes ■ High throughput-lysis reaction can be completed in 96-well PCR plateApplication: genetically modified identification, genotyping, etc. Sample: plant leaf Dosage: Diameter 2-3mm (direct method), diameter 5-7mm (lysis method) Detection range: target fragment ≤1kbBased on Plant Leaf Direct PCR Plus kit, dUTP is used instead of dTTP, and the UNG enzyme that can degrade the template containing dUTP is added. In this way, the specificity and accuracy of amplification are ensured, and the problem of PCR product contamination that may occur during large-scale genetic testing is prevented.  

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