Plant leaf Direct PCR Plus kit (without Sampling Tools) Protocol Direct PCR from Plant Material

Directly use polysaccharide and polyphenol leaf lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity. No time-consuming and expensive DNA purification(Time, labor and cost saved!) Less material Simple—the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding 2 X Mix—reducing sample loading errors and reaction system preparation time Fast—template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes High throughput—lysis reaction can be completed in 96-well PCR plate foregene strength      

Products Details

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Part I Buffer PS1
Buffer P2
6×DNA Loading Buffer
Part II Buffer PS2
2× Leaf PCR EasyTM Mix
■ No time-consuming and expensive DNA purification ■ Less material ■ Simple-the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding ■ 2 × Mix, reducing sample loading errors and reaction system preparation time ■ Fast-template preparation can be completed in 10 minutes, PCR reaction can be completed in at least 50 minutes ■ High throughput-lysis reaction can be completed in 96-well PCR plateApplication: Identification of transgenic plants, genotyping, etc. Sample: plant leaves rich in polysaccharide or polyphenol Sample size: Diameter 2-3mm (direct method), diameter 5-7mm (lysis method) Detection range: PCR product ≤1kbThis product uses a unique lysis buffer system to lyse plant leaves. The lysate can be used as a template without purification. After adding the primers, the PCR Mix of this kit can be used for amplification.

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