Plant Seed Direct PCR Kit I/II (without Sampling Tools) Protocol Plant Direct PCR Kits

◮No time-consuming and expensive DNA purification(Time, labor and cost saved!) ◮Less material ◮Simple—the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding ◮2 X Mix—reducing sample loading errors and reaction system preparation time ◮Fast—template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes ◮High throughput—lysis reaction can be completed in 96-well PCR plate foregene strength

Products Details

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Plant Seed Direct PCR Kit I

Part I

Buffer SP1

Buffer SP2

6×DNA Loading Buffer

Part II

2×Seed PCR EasyTM Mix


Plant Seed Direct PCR Kit  II

Part I

Buffer SP1

Buffer SP2

6×DNA Loading Buffer

Part II

2×Seed PCR EasyTM Mix

■ No time-consuming and expensive DNA purification ■ Less material ■ Simple—the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding ■ 2X Mix—reducing sample loading errors and reaction system preparation time ■ Fast—template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes ■ High throughput—lysis reaction can be completed in 96-well PCR plate.Application: Identification of transgenic plants, genotyping, etc. Sample: Plant seeds or tissues with low polysaccharide and polyphenol content. Sample size: Single whole seed, 1-100mg plant tissue Detection range: PCR product ≤1kbThis product uses a unique lysis buffer system to lyse plant seeds. The lysate can be used as a template without purification. After adding the primers, the PCR Mix of this kit can be used for amplification.

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