Plant Seed (large and medium-sized) Direct PCR Plus Kit II-UNG(without Sampling Tools)

Directly use polysaccharide polyphenol seed lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity. The UNG anti-pollution system has been added to prevent false positives. No time-consuming and expensive DNA purification is required. The sample demand is small, just take a single seed. No special treatments such as grinding and crushing are required, and the operation is simple. The optimized PCR system enables PCR to have higher specificity and stronger tolerance to PCR reaction inhibitors. foregene strength

Products Details

50T,200T,500T,2000T- No time-consuming and expensive DNA purification. - The sample demand is small, just take a single seed. - No special treatments such as grinding and crushing are required, and the operation is simple. - The optimized PCR system enables PCR to have higher specificity and stronger tolerance to PCR reaction inhibitors. - Anti-pollution PCR system 2× Seed PCR EasyTM Mix (UNG), which can effectively eliminate the pollution caused by PCR products and ensure the specificity and accuracy of amplification.1.Transportation The whole process of low-temperature ice box transportation, to ensure that the kit Part II is in a state of <4 ℃.  2.Storage Part I of this kit should be stored at room temperature or 2-8°C. Part II should be stored at -20°C.This product uses a unique lysis reaction system, which can quickly release genomic DNA from plant seed samples with high polysaccharides and polyphenols (such as bananas, cotton, etc.) for use in PCR reactions, so it is particularly suitable for large-scale genetic testing. In order to meet the needs of different detection tests, this kit can use multiple types of samples (such as whole seeds, small tissue cuts, or ground samples of multiple seeds) for experiments. Among them, for the seed samples that still need to be germinated, you can choose to cut out micro-tissue pieces (1-5mg) other than the seed embryos for experiments; for experiments that require sampling and testing in a large number of samples, you can choose to mix multiple samples and combine them. After grinding into particles with a diameter of about 0.5mm, the experiment can be carried out (at least 0.1% of the target sample can be detected).

Plant Seed Direct PCR Plus Kit II-UNG

Polysaccharide polyphenol plant seed (large and medium-sized) direct PCR kit-UNG

 Kit composition

TP-0318T TP-03181

TP-03182

TP-03183
50 T 200 T 500 T 2000 T
  Part I Buffer SSP1 24ml 96ml

120ml×2

480ml×2
Buffer SP2 3ml 10ml 25ml 100ml
6× DNA Loading Buffer 1.5ml 1.5ml 1.5ml 1.5ml×4
  Part II Buffer SSP2 8ml 32ml 80ml 80ml×4
2× Seed PCR EasyTM Mix 500μl 1ml×2

1.7ml×3

1.7ml×12
Manual 1 1 1

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