The entire system does not contain RNase, so the purified RNA will not be degraded. Buffer PRW1 and Buffer PRW2 can ensure that the RNA obtained is not contaminated by protein, DNA, ions, and organic compounds.
■ Operation at room temperature (15-25℃) throughout the whole process, without ice bath and low temperature centrifugation. ■ Complete kit RNase-Free, no need to worry about RNA degradation. ■ Particularly suitable for the purification of RNA from plant samples of polysaccharides and polyphenols. ■ DNA-Cleaning Column specifically binds to DNA, so that the kit can remove genomic DNA contamination without adding DNase. ■ High RNA yield: RNA-only Column and unique formula can efficiently purify RNA. ■ Fast speed: easy to operate and can be completed within 30 minutes. ■ Safety: no organic reagent is required. ■ High quality: The purified RNA fragments are of high purity, free of protein and other impurities, and can meet various downstream experimental applications.■ Downstream applications: first-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, etc. ■ Sample: Fresh or frozen plant tissues of polysaccharides and polyphenols ■ Dosage: 50mg plant tissue ■ Maximum RNA binding capacity of purification column: 80 μg ■ Elution volume: 50-200 μlIt is suitable for the extraction and purification of total RNA from fresh or frozen plant tissue samples (especially fresh plant leaf tissue) with high polysaccharide and polyphenol content.
Plant Total RNA Isolation Kit Plus processed 50mg of fresh leaves of polysaccharides and polyphenols, and 5% purified RNA was tested by electrophoresis. 1: Banana 2: Ginkgo 3: Cotton 4: Pomegranate| Buffer PSL1, Buffer PS, Buffer PSL2 |
| Buffer PRW1, Buffer PRW2 |
| RNase-Free ddH2O, DNA-Cleaning Column |
| RNA-Only Column |