100 T (20μl system) 500 T (20μl system) | RT-qPCR EasyTM (One Step)-SYBR Green I |
| Kit Contents | RT-02111 | RT-02112 |
| 100T (20μl system) | 500T (20μl system) |
| 2× RT-qPCR EasyTM Mix-SYBR | 1ml | 1.7ml×3 |
| 50× ROX Reference Dye | 200μl | 1ml |
| RNase-Free ddH2O | 1.7ml | 1.7ml×3 |
| Instruction Manual | 1piece | 1 piece |
- One-step kit makes reverse transcription and qPCR two reactions in the same tube, only need to add template RNA, specific PCR primers and RNase-Free ddH2O. - The kit can quickly and efficiently quantitatively analyze viral RNA or trace RNA. - The kit uses a unique Foregene reverse transcription reagent and Foregene HotStar Taq DNA Polymerase combined with a unique reaction system to effectively improve the amplification efficiency and specificity of the reaction. - The optimized reaction system makes the reaction have higher detection sensitivity, stronger thermal stability, and better tolerance. - RT-qPCR EasyTM (One Step)-SYBR Green I kit comes with ROX internal reference dye, which can be used to eliminate signal background and signal errors between wells, which is convenient for customers to use in different models of quantitative PCR instruments.
1. Transportation conditions
The whole process of low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃. 2. Storage conditions The kit is stored at -20℃. Store the product in a constant temperature refrigerator at -20℃ immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period. RT-qPCR EasyTM (One Step)-SYBR Green I uses a unique system to effectively combine RT and Real Time PCR reactions, which can quickly complete the quantitative detection of genes. The product has strong tolerance, high specificity and stability. The product contains the company's unique Foregene HotStar Taq DNA Polymerase, which has the advantages of faster amplification (2Kb/min), high amplification efficiency, strong specific amplification ability, and low mismatch rate compared with ordinary Taq enzymes. It is used here for Real Time RT-PCR to reduce non-specific amplification and improve the accuracy of PCR.
