SARS-CoV-2 Variant Nucleic Acid Detection Kit III (Multiplex PCR Fluorescent Probe Method)-for detection of Delta mutants

◮ Direct PCR: No need for nucleic acid extaction kit and System, completed 96 specimen in 1 hour or so. ◮ Low equipment requirement and highly flexible use:Only need Real-Time PCR system . ◮ LoD and high sensitivity:Detection sensitivity can reach the lowest level as 500 copies/ml. foregene strength

Products Details

48 Rxns/kit, 96 Rxns/kit

No.

Component

Amount

Main ingredient

48 Rxns

96 Rxns

1

Nucleic acid release agent

1.4 mL/ tube

2 tubes

5.3 mL/bottle

1 bottle

Surfactant

2

RNA protectant

53 μL/tube

1 tube

106 μL/tube

1 tube

RNase inhibitor

3

SARS-CoV-2 Variant

reaction solution A

800 μL/tube

1 tube

1600 μL/tube

1 tube

Primer, probe, reaction buffer, dNTP

4

SARS-CoV-2 enzyme mixture

160 μL/tube

1 tube

320 μL/tube

1 tube

Hot start Taq enzyme, M-MLV enzyme

5

SARS-CoV-2

positive control

100 μL/tube

1 tube

100 μL/tube

1 tube

Recombinant plasmid containing target fragment, RNA

6

SARS-CoV-2 negative control

1200 μL/tube

1 tube

1200 μL/tube

1 tube

TE buffer

Note:Components from different kits cannot be used interchangeably.
1.Applicable instrument

ABI7500,Bio-Rad CFX96,Roche LightCycler 480,SLAN-96S

2.Detection procedure

Sample processing:Naopharyngeal or oropharyngeal swab sample (only 1 step operation)(Wait for 10 mins)

PCR amplificaton(<=55min)

SARS-CoV-2 SARS-CoV-3 SARS-CoV-4Quick and easy:complete the test in about 1 hour Avoid nucleic acid loss:no need for nucleic acid purification Low detection limit and high sensitivity, especially suitabe for large-scale rapid detection Low equipment requirement and highly flexible use Detection sensitivity can reach the lowest level as 500 copies/mL).Sealed from light and stored at -20±5℃; Store at 2-8℃ after opening, use within 7 days; avoid repeated freeze-thaw(<5 times) Shelf life:1 year

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